It will be the main event of what is expected to be UFC 151, one of two pay-per-views set for September.
The 24-year-old Jones (16-1) will be looking for the fourth successful defense of the title he won in March 2011 by defeating Mauricio Rua. He's won seven in a row, defeating rival Rashad Evans by unanimous decision several weeks ago at UFC 145.
Henderson (29-8) essentially earned the shot with a unanimous decision classic over Rua last November at UFC 139. Before that, he was the last Strikeforce Light Heavyweight Champion, taking the title from Rafael Cavalcante last March but never defending the gold. The 41-year-old has won four in a row and notably finished Fedor Emelianenko last July in Strikeforce.